Part:BBa_K1997013:Design

P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 382
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 624
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1003
  Illegal AgeI site found at 1087
  Illegal AgeI site found at 1207
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1210
  Illegal BsaI.rc site found at 907
  Illegal SapI.rc site found at 1324
  

Design Notes

Two restriction sites for Gibson assembly was added at the flank of "Zif-N-RBS-Zif-C" sequence, so that such sequence could be sassily replaced.

Source

This part was synthesized by company